Effects of tonsillectomy combined with suspension sutures on adult obstructive sleep apnea-hypopnea syn-drome / 实用医学杂志

Objective To investigate the therapeutic benefits of tonsillectomy combined with suspension sutures on Friedman stageⅠ obstructive sleep apnea-hypopnea syndrome(OSAHS). Methods Forty-four pa-tients with Friedman stageⅠOSAHS diagnosed and treated from January 2014 to December 2016 were divided into experimental group with 23 patients receiving tonsillectomy combined with suspension sutures and the control group with 21 receiving modified uvulopalatopharyngoplasty. Apnea-hypopnea index(AHI),lowest oxygen saturation (LSaO2)and Epworth Sleepiness Scale(ESS)score were compared between the groups pre- or post-operatively. Results Surgical success rate did not differ significantly between 2 groups(82.6% in the experimental group and 80.9% in the control group). Inner-group comparison indicated that AHI and ESS were significantly lower(P <0.001)while LSaO2was significantly higher after the operation(P < 0.001). There were,however,no significant differences in pre-or post-operative BMI,AHI,ESS or LSaO2between the experimental and control group(all P≥0.05).Conclusion Tonsillectomy combined with suspension sutures is effective for Friedman stageⅠOSAHS.

Factors Influencing Facial Profile Evaluation / 中国医科大学学报

Objective To characterize differences within facial profile esthetic evaluations among three groups of evaluators and the influences of sagittal or vertical skeletal patterns on facial profile esthetics. Methods A total of 114 silhouettes,from the pre?or post?treatment facial profiles of 57 patients,were scored by three groups of evaluators(professionals,patients'parents,and laypersons)to characterize differences within facial es?thetics evaluation among evaluators. The highest?scoring 30 silhouettes and the lowest?scoring 30 silhouettes were selected as the high score group and the low score group,respectively. Sixteen indices of the relative lateral cephalometric films were measured. Data were analyzed by SPSS 21.0. Results Facial profiles following orthodontic treatment scored higher than pre?treatment(P<0.05). There were significant differences between professionals and laypersons in facial profile scoring. Different sagittal skeletal patterns had no effect on esthetic evaluation ,but lower facial height significantly influenced whether a patient received a high or low esthetic score(P<0.05). Conclusion Orthodontic treatment can improve the fa?cial profile significantly,and lower facial height is the most sensitive index for facial profile evaluation in our study. However,all three evaluator groups have different esthetic standards that don't overlap. Notably,the expectations of laymen are higher than those of orthodontists.

Effect of methotrexate packaged by tumor derived microparticles combined with radiotherapy on proliferation of cancer stem cells in lung adenocarcinoma in vitro / 肿瘤研究与临床

Objective To explore the effect of methotrexate packaged by tumor derived microparticles (T-MP MTX) combined with radiotherapy on lung cancer stem cell (CSC) in vitro. Methods T-MP MTX was prepared from non-small cell lung cancer A549 cells. Proliferative changes of A549 cells, bronchial epithelial cells H460 and 16HBE cells treated by T-MP MTX were assayed by MTT method. Cell cycles of A549 cells in blank group and T-MP MYX group were examined by fluorescence activated cell sorting (FACS). The effect of T-MP MTX combined with radiotherapy on CSCs was assessed by tumor sphere formation experiment and animal experiment. The expressions of stemness relative genes (such as β-catenin, Nanog, SOX-2 and KLF4) were measured by Western blot. Results T-MP MTX dose-dependently inhibited the cell growth in A549 cells, but didn't in H460 cells and 16HBE cells. The S cycle ratio of A549 cells in blank group and T-MP MYX group measured by FACS were (15.83±3.14)%and (47.47±6.69)%, respectively. S cycle ratio of T-MP MYX group was notably higher compared with that of blank group (t=7.411, P=0.002). Further study revealed that the number of tumor sphere in blank group, control group, 2 Gy group, 4 Gy group and 6 Gy group was (268.9±22.4), (172.4±18.7), (48.3±5.1), (16.3±3.5) and (5.1±3.1), respectively. The number of tumor sphere in other groups was decreased compared with that in blank group (F=228.291, P=0.000). The numbers of tumor sphere in 2 Gy group, 4 Gy group and 6 Gy groups was also reduced compared with that in control group. Importantly, the number of tumor sphere in these groups were decreased dramatically as the dose of radiotherapy increased (F=95.142, P=0.000). The results of tumor sphere volume were similar with the number of tumor sphere. Western blot experiment showed that T-MP MTX treatment in A549 cells decreased the expression of stemness relative genes (β-catenin, Nanog, SOX-2 and KLF4), and its role was reinforced when radiotherapy was combined. Animal experiment implied that activity of luciferase in T-MP MTX group was decreased compared with that in blank group (P=0.000), and the activity of luciferase in T-MP MTX plus 2 Gy group was reduced significantly (t=6.887, P=0.002). Conclusions T-MP MTX has a potential to sensibilize radiotherapy, and it will synergistically inhibit the proliferation of CSCs when combined with radiotherapy. Moreover, its mechanism may be related with T-MP MTX activating CSCs from hypometabolism state and blocking process of cell cycle.

Analysis the relationship of mean platelet volume and obstructive sleep apnea and hypopnea syndrome / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#Uur aim was to investigate the association between OSAHS and mean platelet volume (MPV) value.@*METHOD@#This study included 70 cases with OSAHS in our ward between Jan. 2012 and Jan. 2014, and the OSAHS patients were divided two groups: mild- to moderate group and severe group; 30 age-and sex-matched healthy subjects was in control group. The correlation among the levels of the number of platelets (PLT), MPV, platelet distribution width (PDW) were evaluated in the two groups.@*RESULT@#PLT count was significantly lower in the severe group than the control group [(202. 8 ± 68. 9] × 10(9)/L, (235. 9 ± 65. 2) × 10(9)/L]; MPV and PDW were significantly higher in the severe group [(10. 9 ± 0. 9), (10. 4±0. 8) fL; (12. 9 ± 1. 9) %, (12. 0 ± 1. 4) %]. There was not significantly difference of MPV between the mild to moderate group and the control group [(10. 7 ± 0. 7), (10. 4 ± 0. 8)fL].@*CONCLUSION@#MPV levels are elevated in patients with the severe OSAHS.

Analysis the relationship of mean platelet volume and nasal obstructive disease / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#High mean platelet volume (MPV) values are a risk factor for cardiovascular diseases. Our aim was to investigate the association between nasal obstructive disease and MPV value.@*METHOD@#This study included 40 patients with nasal obstructive disease and 30 age-and sex-matched healthy subjects as controls. The levels of the number of platelets, MPV, platelet distribution width (PDW) were evaluated and compared in the two groups. Then the MPV was compared in the patients before and after operation in the study group.@*RESULT@#Platelet count is significantly lower in the study group than the control group [(215.0 ± 47.5) x 10(9)/L versus (237.8 ± 46.2) x 10(9)/L]; MPV and PDW is significantly higher in the study group [(10.7 ± 0.7) fL versus (10.3 ± 0.8) fL]; [(12.9 ± 2.0) % versus (12.0 ± 1.3) %]. Comparing to preoperation, MPV values of the study group is significantly decreased after nasal obstructive operations [(10.7 ± 0.7) fL versus (10.4 ± 0.6) fL].@*CONCLUSION@#MPV levels are elevated in patients with nasal obstructive disease, and can be decreased by nasal obstructive operation.

The ReIationship of Mean PIateIet VoIume and Sudden SensorineuraI Hearing Loss / 听力学及言语疾病杂志

Objective Our aim was to investigate the association between sudden sensorineural hearing loss (SSHL)and mean platelet volume (MPV)values,in order to evaluate the etiology of SSHL.Methods This study included 40 patients with SSHL in our ward between Jan.2012 and Jan.2014 and 30 age- and sex-matched healthy subjects were included as controls.The correlation among the levels of the number of platelets,mean plate-let volume(MPV),platelet distribution width (PDW)was evaluated in the two groups.ResuIts The platelet count was significantly lower in the SSHL than the control group(212.0±47.9 versus 237.8±46.2 ×109/L).MPV and PDW were significantly higher in SSHL(10.9±0.8 versus 10.3±0.8 fL;12.9%±2.0% versus 12.0%±1.3 %). The SSHL patients were divided two subgroups:the mild-moderate subgroup (hearing loss 25~70 dB)and the se-vere subgroup(hearing loss≥70 dB).The MPV values were insignificantly different between the two subgroups(11. 0±1.1 fL versus 10.8 ±1.0 fL).Compared to the control group,the two subgroups had both significant differ-ences in MPV.ConcIusion High MPV values are a risk factor for thrombotic vascular diseases.Considering the in-creased MPV levels,SSHL appears to be characterized by ischaemic or thrombotic events.

Growth and mutation of Escherichia coli with suicide gene circuit based on quorum sensing / 生物工程学报

Constructing robust gene circuits is a fundamental work for synthetic biology. Bacteria with suicide gene circuit based on quorum-sensing will kill themselves in a controllable pattern upon certain cell density. In the media of different IPTG inducer concentration, we observed the growth and suicidal behavior of the Escherichia coli. Top10F' with such gene circuit, screened the mutants and determined their mutated loci. The results show that, with higher IPTG concentration, the more wild type bacteria were killed; as well the mutants emerged earlier and spread over the population more quickly. The sequence of plasmids in those mutants revealed that a transposon inserted into the luxR gene and therefore disrupted Quorum-Sensing of these individuals. Furthermore, the insertion sequence of the plasmid can solely result in the mutants escaping from suicide.

Influence of Shenmai injection on immune function in the treatment of locally advanced non-small cell lung cancer patients with concurrent chemoradiotherapy / 中国基层医药

Objective To observe the effect of Shenmai injection combined concurrent chemoradiotherapy on immune function in patients with locally advanced non-small cell lung cancer.Methods A total of 80 patients with locally advanced non-small cell lung cancer were randomly divided into treatment group and control group.In treatment group,40 patients received simultaneous three-dimensional conformal radiotherapy combined with TP(docetaxel+cisplatin)chemotherapy,while given Shenmai injection in the process of concurrent chemoradiotherapy.In control group,40 patients only received chemoradiotherapy.The changes of T cell subsets were observed between the two groups.Results It showed no significant differences in percentages of CD+3,CD+4,CD+16CD+56and CD+4/CD+8 ratio between two groups pre-treatment(all P＞0.05).There were no significant adverse reactions occurred after received Shenmai injection in treatment group.Conclusion It play an important role in improving immune function of patients with locally advanced non-small cell lung cancer who received Shenmai injection combined concurrent chemoradiotherapy.

The severity of obstructive sleep apnea and hypopnea syndrome in different sleep stage / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#This study aimed to inspect the hypothesis that obstructive sleep apnea and hypopnea syndrome (OSAHS) was commonly worse in REM sleep than in NREM sleep stage, and also to analyze the relevance with the risk factors of OSAHS.@*METHOD@#One hundred eighteen patients diagnosed as OSAHS by overnight polysomnography (PSG) were studied retrospectively. The severity of OSAHS in REM versus NREM sleep was compared. Then the patients were divided into two groups: AHI(REM) > or = AHI(NREM) group, and AHI(REM) < AHI(NREM) group, and the two groups were compared in age, sex, BMI, AHI, AHI supine, oxygen desaturation and duration of apnea.@*RESULT@#There was no significant difference between AHI(REM) and AHI(NREM). AHI(REM)/AHI(NREM) was 1.0 +/- 0.5. A higher AHI NREM than AHI REM group was found in 55.9% of the 118 patients with OSAHS. There were no differences between the clinical and PSG founding of two groups.@*CONCLUSION@#The severity of OSAHS is not greatly influenced by sleep stage.

Clinical features of OSAHS closely associated with supine position / 中国耳鼻咽喉头颈外科

OBJECTIVE To study the clinical features of OSAHS patients closely associated with supine position. METHODS The clinical data of 105 patients with OSAHS diagnosed by overnight polysomnography(PSG)were retrospectively studied. The patients were divided in two groups: the positional patients(PP)group, those patients who had a apnea-hypopnea index(AHI) that was at least two times higher than the lateral AHI, and the non-positional patients(NPP)group, those patients in whom the AHI in the supine position was two times less than that in the lateral position, and the two groups were compared with age, BMI and the severity of OSAHS. RESULTS The difference of age and BMI were not significant between the two groups[(40.4?10.5)year versus(37.0?10.6) year,(28.2?3.8)kg/m2 versus(29.1?3.0)kg/m2]. There was significantly different in AHI between two groups[(36.8?25.4)/h versus (51.2?29.0)/h ]. AHIsupine and AHInot-supine was significantly different in mild and moderate OSAHS patients [(23.9?13.8) /h versus(12.4?10.1)/h ]. CONCLUSION Mild and moderate OSAHS patients are mainly PP. Positional therapy should play an important role in treatment of mild and moderate patients.